IL-6/IFN-β2/BSF-2, Human, Recombinant
英文名稱:IL-6/IFN-β2/BSF-2, Human, Recombinant
貨號: PCK085
價格: ¥9680 ¥2780 ¥880 ¥13880
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 500μg / 50μg / 10μg / 1mg |
別名 | IFN-β2;B-Cell Differentiation Factor (BCDF);BSF-2;HPGF;HSF;MGI-2 |
物種 | 人 |
表達(dá)宿主 | E.coli |
序列信息 | MVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM with polyhistidine tag at the C-terminus. |
檢索號 | P05231.1 |
分子量 | 21.8 kDa |
表觀分子量 | 22 kDa |
標(biāo)簽 | His-tag at the C-terminus |
生物活性 | Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately > 5 × 10^8 IU/mg.Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is < 4.4 ng/mL. |
純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
內(nèi)毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
運輸條件 | Ambient temperature or ice pack. |
有效期 | 12個月 |
制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹 | Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. |
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期刊:HUMAN & EXPERIMENTAL TOXICOLOGY
影響因子:2.7
引用產(chǎn)品: GM-CSF/CSF2 (C-6His), Mouse, Recombinant, 特級胎牛血清, IL-6/IFN-β2/BSF-2, Human, Recombinant, 丙酮酸鈉溶液(100 mM),100×
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期刊:JOURNAL OF CLINICAL LABORATORY ANALYSIS
影響因子:3.124
引用產(chǎn)品: SCF/c-Kit/KIT ligand, Human, Recombinant, IL-6/IFN-β2/BSF-2, Human, Recombinant, 293T [HEK-293T] 細(xì)胞, 人皮膚肥大細(xì)胞完全培養(yǎng)基
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Q1:官網(wǎng)的因子都是不含動物成分的么?
我們公司的因子都是重組的,是不含動物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液,用于實驗時,需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?還是需要通過PBS(含5%海藻糖)進(jìn)一步稀釋才行呢?
如果是直接做實驗,可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話,需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎?
加了無菌水后,如果沒有掛壁現(xiàn)象是不用離心的,如果您放置了一段時間,再次使用時是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎?
不用提前放置室溫.
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Q5:因子到貨了,里面有兩管溶液,一個是水,一個是海藻糖溶液,用哪一個稀釋呢?
1、將裝有細(xì)胞因子的管子放入小離心機(jī)內(nèi)短暫離心20~30s(離心機(jī)最大轉(zhuǎn)速即可),使附著在管壁的微量蛋白聚集在管底; 2、先用無菌水溶解因子,很少量水即可溶解因子,注意計算溶解后濃度不低于100 μg/mL; 3、用贈送的含海藻糖的PBS溶液進(jìn)一步稀釋到您實驗合適的濃度即可,然后分裝后放入-20℃以下保藏; 4、因子復(fù)融重懸過程應(yīng)輕柔,不可以震蕩渦旋。
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Q6:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融?
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨,少部分液體形式發(fā)貨; 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶,請參考產(chǎn)品說明書【復(fù)融】方法,按照要求使用對應(yīng)溶劑溶解,大部分因子為無菌水復(fù)融,少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會贈送相應(yīng)的復(fù)融試劑;凍干粉溶解后如您需要進(jìn)一步稀釋分裝,需使用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶,具體濃度詳見產(chǎn)品標(biāo)簽,按照實驗需要用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可;

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