GM-CSF/CSF2 (C-6His), Mouse, Recombinant
英文名稱:GM-CSF/CSF2 (C-6His), Mouse, Recombinant
貨號(hào): PCK039
價(jià)格: ¥11880 ¥3480 ¥1180 ¥18880
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 500μg / 50μg / 10μg / 1mg |
別名 | Granulocyte-Macrophage Colony-Stimulating Factor;GM-CSF;Colony-Stimulating Factor;CSF;Molgramostin;Sargramostim;CSF2;GMCSF |
物種 | 小鼠 |
表達(dá)宿主 | Human Cells |
序列信息 | Ala18-Lys141 |
檢索號(hào) | P01587 |
分子量 | 15.1 kDa |
表觀分子量 | 18-30 kDa |
標(biāo)簽 | C-6His |
生物活性 | Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is 40-170 pg/mL. |
純度 | > 95% as determined by reducing SDS-PAGE. |
內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
運(yùn)輸條件 | Ambient temperature or ice pack. |
有效期 | 12個(gè)月 |
制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介紹 | Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a Growth Factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to Cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a Growth Factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines. |
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期刊:HUMAN & EXPERIMENTAL TOXICOLOGY
影響因子:2.7
引用產(chǎn)品: GM-CSF/CSF2 (C-6His), Mouse, Recombinant, 特級(jí)胎牛血清, IL-6/IFN-β2/BSF-2, Human, Recombinant, 丙酮酸鈉溶液(100 mM),100×
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么?
我們公司的因子都是重組的,是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液,用于實(shí)驗(yàn)時(shí),需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?還是需要通過PBS(含5%海藻糖)進(jìn)一步稀釋才行呢?
如果是直接做實(shí)驗(yàn),可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話,需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎?
加了無菌水后,如果沒有掛壁現(xiàn)象是不用離心的,如果您放置了一段時(shí)間,再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎?
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融?
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨,少部分液體形式發(fā)貨; 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶,請(qǐng)參考產(chǎn)品說明書【復(fù)融】方法,按照要求使用對(duì)應(yīng)溶劑溶解,大部分因子為無菌水復(fù)融,少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會(huì)贈(zèng)送相應(yīng)的復(fù)融試劑;凍干粉溶解后如您需要進(jìn)一步稀釋分裝,需使用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶,具體濃度詳見產(chǎn)品標(biāo)簽,按照實(shí)驗(yàn)需要用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可;

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