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HFL1 (人胚肺成纖維細(xì)胞) (STR鑒定正確)
貨號(hào)：CL-0106
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價(jià)格： ￥1600
生長培養(yǎng)基：Ham's F-12K＋10%FBS＋1% P/S
產(chǎn)品概述
| 名稱 | HFL1 (人胚肺成纖維細(xì)胞) (STR鑒定正確) |
| 別稱 | HFL-1; HFL 1; Human fetal lung fibroblast 1; HFL |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 成纖維細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項(xiàng) | 該細(xì)胞為有限傳代細(xì)胞系，傳代次數(shù)過高會(huì)導(dǎo)致細(xì)胞生長特性發(fā)生變化，建議到貨3代內(nèi)完成實(shí)驗(yàn)。 |
| 年齡（性別） | 男性；胎兒 |
| 組織來源 | 肺 |
| 細(xì)胞類型 | 有限細(xì)胞系 |
| 生物安全等級(jí) | BSL-1 |
| 保藏機(jī)構(gòu) | ATCC; CCL-153 BCRC; 60299 ECACC; 89071902 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 17,25 D3S1358 14,17 D5S818 12 D7S820 9,10 D8S1179 12,14 D13S317 11,12 D16S539 9,11 D18S51 18,19 D19S433 11,13 D21S11 27,30 FGA 21,22 PentaD 2.2,9 PentaE 12,20 TH01 7,9 TPOX 6,9 vWA 17 D6S1043 11,18 D12S391 20,21 D2S441 14,15 -
STR鑒定圖
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參考文獻(xiàn)
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