TGFB1/TGFβ1/TGF-β1, Human, Recombinant
英文名稱(chēng):TGFB1/TGFβ1/TGF-β1, Human, Recombinant
貨號(hào): PCK091
價(jià)格: ¥14680 ¥6280 ¥3480 ¥1180 ¥23880
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 500μg / 100μg / 50μg / 10μg / 1mg |
別名 | Transforming Growth Factor Beta-1;TGF-Beta-1;Latency-Associated Peptide;LAP;TGFB1;TGFB |
物種 | 人 |
表達(dá)宿主 | CHO Stable Cells |
序列信息 | Ala279-Ser390 |
檢索號(hào) | P01137 |
分子量 | 12.8 kDa |
表觀分子量 | 13 kDa |
生物活性 | Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/mL. |
純度 | > 95% as determined by reducing SDS-PAGE. |
內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
運(yùn)輸條件 | Ambient temperature or ice pack. |
有效期 | 12個(gè)月 |
制劑 | Lyophilized from a 0.2 μm filtered solution of 50 mM Glycine-HCl, 150 mM NaCl, 4% Mannitol, pH 2.5. |
復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介紹 | Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease. |
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期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-025-03337-7
影響因子:10.6
引用產(chǎn)品: TGFB1/TGFβ1/TGF-β1, Human, Recombinant
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期刊:Chemical Biology & Drug Design
影響因子:3.2
引用產(chǎn)品: 293A 細(xì)胞, DMEM無(wú)糖 培養(yǎng)基, DMEM高糖 培養(yǎng)基, 特級(jí)胎牛血清, TGFB1/TGFβ1/TGF-β1, Human, Recombinant
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期刊:CELL BIOCHEMISTRY AND BIOPHYSICS
DOI:10.1007/s12013-025-01668-8
影響因子:1.8
引用產(chǎn)品: TGFB1/TGFβ1/TGF-β1, Human, Recombinant, THLE-2 細(xì)胞
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期刊:Regenerative Therapy
DOI:10.1016/j.reth.2024.05.002
影響因子:4.3
引用產(chǎn)品: HGF R/c-Met/HGFR (Glu25-Gly519), Human, Recombinant, TGFB1/TGFβ1/TGF-β1, Human, Recombinant
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期刊:Cancer Medicine
影響因子:4.0
引用產(chǎn)品: TGFB1/TGFβ1/TGF-β1, Human, Recombinant, MRC-5 細(xì)胞
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期刊:Annals of Translational Medicine
影響因子:3.616
引用產(chǎn)品: TGFB1/TGFβ1/TGF-β1, Human, Recombinant
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期刊:Reproductive Biology
DOI:10.1016/j.repbio.2022.100701
影響因子:2.100
引用產(chǎn)品: TGFB1/ TGFβ1/ TGF-β1, Human, Recombinant
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SMAD4 Inhibits Granulosa Cell Apoptosis via the miR-183-96-182 Cluster and FoxO1 Axis(2021)
期刊:Reproductive Sciences
DOI:10.1007/s43032-021-00690-4
影響因子:2.900
引用產(chǎn)品: TGFB1/ TGFβ1/ TGF-β1, Human, Recombinant
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期刊:Life Sciences
影響因子:6.100
引用產(chǎn)品: TGFB1/ TGFβ1/ TGF-β1, Human, Recombinant
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么?
我們公司的因子都是重組的,是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無(wú)菌水配成了100ug/mL母液,用于實(shí)驗(yàn)時(shí),需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?zhuān)窟€是需要通過(guò)PBS(含5%海藻糖)進(jìn)一步稀釋才行呢?
如果是直接做實(shí)驗(yàn),可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話(huà),需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無(wú)菌水溶解后需要再次離心嗎?
加了無(wú)菌水后,如果沒(méi)有掛壁現(xiàn)象是不用離心的,如果您放置了一段時(shí)間,再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎?
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融?
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨,少部分液體形式發(fā)貨; 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶,請(qǐng)參考產(chǎn)品說(shuō)明書(shū)【復(fù)融】方法,按照要求使用對(duì)應(yīng)溶劑溶解,大部分因子為無(wú)菌水復(fù)融,少部分需要無(wú)菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會(huì)贈(zèng)送相應(yīng)的復(fù)融試劑;凍干粉溶解后如您需要進(jìn)一步稀釋分裝,需使用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶,具體濃度詳見(jiàn)產(chǎn)品標(biāo)簽,按照實(shí)驗(yàn)需要用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可;

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