M-CSF/CSF1, Mouse, Recombinant
英文名稱：M-CSF/CSF1, Mouse, Recombinant
貨號(hào)： PCK043
價(jià)格： ￥12880 ￥6280 ￥3480 ￥1180 ￥18880
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 別名 | Macrophage colony-stimulating factor 1;CSF-1;MCSF;Csf1;Csfm |
| 物種 | 小鼠 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Lys33-Glu262 |
| 檢索號(hào) | P07141 |
| 分子量 | 26.0 kDa |
| 表觀分子量 | 37-80kDa |
| 生物活性 | Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.04-0.2 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個(gè)月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Macrophage colony-stimulating factor 1 (M-csf) is a single-pass type I membrane Protein. It is a hematopoietic Growth Factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane Receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. |
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期刊：BIOCHEMICAL PHARMACOLOGY
影響因子：5.3
引用產(chǎn)品： M-CSF/CSF1, Mouse, Recombinant
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期刊：Advanced Biology
影響因子：3.2
引用產(chǎn)品： M-CSF/CSF1, Mouse, Recombinant, 青霉素-鏈霉素溶液（雙抗），100×, 小鼠骨髓間充質(zhì)干細(xì)胞完全培養(yǎng)基, 特級(jí)胎牛血清, RAW 264.7 細(xì)胞, 小鼠骨髓間充質(zhì)干細(xì)胞, DMEM高糖 培養(yǎng)基 , 小鼠骨髓間充質(zhì)干細(xì)胞成骨誘導(dǎo)分化培養(yǎng)基
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期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-022-01614-3
影響因子：9.429
引用產(chǎn)品： RPMI-1640 培養(yǎng)基, M-CSF/CSF-1/MGI-IM, Human, Recombinant
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:NFS-60細(xì)胞完培中的M-CSF因子，可以用PCK043這款嗎
可以。
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Q3:細(xì)胞因子用無(wú)菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過(guò)PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q4:細(xì)胞因子加無(wú)菌水溶解后需要再次離心嗎？
加了無(wú)菌水后，如果沒(méi)有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q5:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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Q6:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融？
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨，少部分液體形式發(fā)貨； 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶，請(qǐng)參考產(chǎn)品說(shuō)明書【復(fù)融】方法，按照要求使用對(duì)應(yīng)溶劑溶解，大部分因子為無(wú)菌水復(fù)融，少部分需要無(wú)菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會(huì)贈(zèng)送相應(yīng)的復(fù)融試劑；凍干粉溶解后如您需要進(jìn)一步稀釋分裝，需使用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶，具體濃度詳見產(chǎn)品標(biāo)簽，按照實(shí)驗(yàn)需要用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可；
識(shí)別碼示意圖
