FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
英文名稱：FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
貨號(hào)： PCK005
價(jià)格： ￥5080 ￥2180 ￥1180 ￥580 ￥7480
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 液體 |
| 產(chǎn)品規(guī)格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 別名 | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | Met1-Ser155 |
| 檢索號(hào) | BAG70135.1 |
| 分子量 | 17.2kDa |
| 表觀分子量 | 17kDa |
| 生物活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.75 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 運(yùn)輸條件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6個(gè)月 |
| 制劑 | Supplied as a 0.2 μm filtered solution of 20 mM Citrate, 10% Trehalose, 150 mM NaCl, 0.04% PS80, 0.5 mM EDTA, pH 5.5. |
| 背景介紹 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
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期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影響因子：2.500
引用產(chǎn)品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
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期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影響因子：5.600
引用產(chǎn)品： PC-9 細(xì)胞, A549 [A-549] 細(xì)胞, NCI-H1299 細(xì)胞, NCI-H1975 細(xì)胞, BEAS-2B 細(xì)胞, RPMI-1640 培養(yǎng)基, BEAS-2B細(xì)胞專用培養(yǎng)基, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融？
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨，少部分液體形式發(fā)貨； 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶，請(qǐng)參考產(chǎn)品說明書【復(fù)融】方法，按照要求使用對(duì)應(yīng)溶劑溶解，大部分因子為無菌水復(fù)融，少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會(huì)贈(zèng)送相應(yīng)的復(fù)融試劑；凍干粉溶解后如您需要進(jìn)一步稀釋分裝，需使用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶，具體濃度詳見產(chǎn)品標(biāo)簽，按照實(shí)驗(yàn)需要用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可；
識(shí)別碼示意圖
