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hCMEC/D3 (人腦微血管內(nèi)皮細(xì)胞) (STR鑒定正確)
貨號(hào)：CL-0843
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價(jià)格： ￥2000
生長(zhǎng)培養(yǎng)基：hCMEC/D3 細(xì)胞專用培養(yǎng)基
產(chǎn)品概述
產(chǎn)品信息
| 名稱 | hCMEC/D3 (人腦微血管內(nèi)皮細(xì)胞) (STR鑒定正確) |
| 別稱 | HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3 |
| 種屬 | 人類 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 紡錘形細(xì)胞 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:6 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來(lái)源文獻(xiàn))
| 背景描述 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
| 年齡（性別） | 女性；成人 |
| 組織來(lái)源 | 腦微血管 |
| 細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
| 生物安全等級(jí) | BSL-2 |
| 保藏機(jī)構(gòu) | KCB; KCB 2019022YJ |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 10,12 D2S1338 16,20 D3S1358 16,17 D5S818 11 D7S820 10,12 D8S1179 15,16 D13S317 11,14 D16S539 9,12 D18S51 12,19 D19S433 12,15 D21S11 30,32.2 FGA 22,26 PentaD 10,12 PentaE 5,10 TH01 7,9 TPOX 10,11 vWA 14,17 D6S1043 17,18 D12S391 19.3,22 D2S441 13 -
STR鑒定圖
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